Human RNF4 ELISA Kit (HUFI08528)

The RNF4 protein, encoded by the RNF4 gene, acts as a SUMO-targeted ubiquitin ligase (STUbL) that recognizes poly-SUMOylated substrates and channels them toward proteasomal turnover. This ELISA Kit from Assay Genie enables quantitative measurement of human RNF4 in research samples, supporting investigation into the post-translational crosstalk between SUMOylation and ubiquitin-dependent protein degradation.

Typical research applications include studies of arsenic-induced PML-RARα clearance in acute promyelocytic leukemia models, DNA double-strand break repair, PML nuclear body maintenance, and STUbL-mediated proteolysis in cancer biology. The kit is suited to in vitro work on cellular stress responses, chromatin-associated signaling, and RNF4-dependent substrate recognition across human cell systems.

Configured as a 96-assay kit, the product ships with pre-coated microplate strips, recombinant calibration standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, substrate solution, and stop reagent needed to complete the run. Detection relies on an enzymatic chromogenic readout measured with a standard microplate absorbance reader capable of recording the appropriate wavelength. The enclosed insert specifies reagent working dilutions, incubation windows, and standard curve preparation steps.

For research use only, this Assay Genie kit serves laboratories studying SUMO-ubiquitin pathway regulation, DNA damage response biology, and targeted protein degradation mechanisms relevant to human disease research.