Human RNF168 ELISA Kit (HUFI08530)

From Assay Genie, this quantitative ELISA targets RNF168, an E3 ubiquitin ligase that orchestrates the chromatin ubiquitination cascade at DNA double-strand breaks. Working alongside RNF8, RNF168 deposits K63-linked ubiquitin chains on histone H2A, generating the docking sites recognized by downstream DNA repair factors 53BP1 and BRCA1. Loss-of-function mutations in this gene underlie RIDDLE syndrome, and altered expression has been reported across multiple cancer types, so quantitative tracking of RNF168 protein has direct value for DNA repair biology, genotoxicity testing, and translational oncology research.

The 96-assay kit is configured for measurement of human RNF168 in clinical sample matrices, supporting translational cohort studies as well as basic research using cultured cells and matched tissue specimens. Applications include monitoring changes in ligase abundance following irradiation or genotoxic drug treatment, profiling tumor versus matched normal tissue, and exploring how E3 ligase dysregulation reshapes the choice between homologous recombination and non-homologous end joining during repair.

The kit is supplied refrigerated on ice packs and individual components should be stored according to the temperature conditions printed on the vial labels. Reagents include pre-coated strips in a break-apart well plate format, lyophilized or liquid standards, detection antibody, and assay buffers; the 8 x 12 strip layout lets researchers run partial plates as needed.