Human RNASEH1 ELISA Kit (HUFI06814)

Ribonuclease H1 (RNASEH1) selectively cleaves the RNA strand of RNA-DNA hybrids and plays indispensable roles in Okazaki fragment processing, mitochondrial DNA replication, and resolution of transcription-associated R-loops. Mutations affecting RNASEH1 function have been linked to chronic progressive external ophthalmoplegia, mitochondrial DNA depletion syndromes, and autoinflammatory conditions resembling Aicardi-Goutières syndrome, making accurate quantification of this enzyme valuable across multiple research areas.

The Assay Genie Human RNASEH1 ELISA Kit is a 96 Assays immunoassay formatted for measurement of RNASEH1 in human research specimens. Each kit includes a pre-coated microplate, calibration standards, biotinylated detection antibody, and streptavidin-HRP conjugate, sufficient for 96 individual determinations. Signal generation follows standard HRP/TMB chemistry, yielding an absorbance readout compatible with conventional microplate readers in life science laboratories.

Applications include studies of HIV-1 reverse transcription, where RNASEH1 contributes to cDNA processing, investigations of R-loop accumulation under conditions of genome instability, and characterization of RNASEH1 expression in cancer cell models. Reagent components should be stored refrigerated between uses, with reconstituted standards handled according to the package insert to preserve binding activity across multiple experimental runs.