Human RIPK2/Receptor TNFRSF Interacting Serine Threonine Kinase 2 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit (Double Antibody) is designed for the quantitative measurement of human RIPK2 (Receptor Interacting Serine/Threonine Kinase 2) across a broad range of sample types, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit uses a colorimetric detection method, with absorbance read at 450 nm following TMB substrate development and a stop reaction.

RIPK2 is a key protein involved in signaling pathways that regulate inflammatory and immune responses, and its dysregulation has been linked to inflammatory disorders, autoimmune diseases, and infections. The assay delivers a sensitivity of 37.5 pg/mL across a measuring range of 62.5-4000 pg/mL, with linearity spanning 81-105% across serum, EDTA plasma and heparin plasma dilutions, and recovery averaging 97% (serum), 98% (EDTA plasma) and 99% (heparin plasma). Reagents should be stored at 2-8°C for 12 months.

The protocol involves equilibrating reagents and TMB substrate, a primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody binding for 60 minutes, HRP-Streptavidin binding for 30 minutes, and TMB colour development in the dark for 10-20 minutes before reading absorbance immediately at 450 nm. The research area is immunology, and the kit targets UniProt entry O43353.