Human RICTOR ELISA Kit (HUFI08536)

RICTOR (Rapamycin-Insensitive Companion of mTOR) is the defining scaffold component of the mTORC2 complex, functionally separate from the rapamycin-sensitive mTORC1 assembly, and it drives phosphorylation of AKT at serine 473 along with SGK1 and PKCα. The Human RICTOR ELISA Kit from Assay Genie provides a quantitative immunoassay to measure RICTOR protein abundance in human research specimens. Altered RICTOR expression is documented in tumor biology, metabolic disorders, immune cell signaling, and cytoskeletal regulation, positioning this protein as a meaningful marker for translational oncology, endocrinology, and cell signaling research.

Provided as a 96-Assay pack for use with a standard microplate workflow, the kit produces a quantitative absorbance readout for curve-based interpolation of unknown sample concentrations. Researchers often pair the assay with cell lysates, tissue homogenates, or matched research samples for comparative analysis of pathway activation under different genetic or pharmacological perturbations. Reagents should be refrigerated at 4°C until first use, and the microplate should be equilibrated to room temperature before initiating the protocol. Protect unused wells and reagents from light and avoid repeated freeze-thaw cycles of diluted standards.

Marked for in vitro research use only, the kit integrates into laboratory workflows investigating mTOR pathway biology, including studies of resistance mechanisms to rapalogs, growth factor signaling, and downstream kinase activity. Compatibility with standard plate readers and standard curve formats supports academic, biotech, and pharmaceutical discovery environments exploring the rapamycin-insensitive arm of mTOR signaling.