Human Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) ELISA Kit

The Assay Genie RPS6KA1 immunoassay provides quantitative measurement of ribosomal protein S6 kinase alpha 1, an ~90 kDa serine/threonine kinase in the p90RSK family and a downstream effector of the MAPK/ERK signaling cascade, in human research samples. RPS6KA1, also known as RSK1, mediates growth-factor-driven proliferation and survival responses and is frequently examined alongside mTOR and Ras/Raf/MEK/ERK pathway activity.

This 96-assay kit is configured for use with tissue homogenates and cell lysates. The microplate is pre-coated with capture antibody specific for the target, and bound analyte is revealed through a biotinylated detection antibody and streptavidin-HRP conjugate. Absorbance is read on a standard plate reader, and the kit supplies pre-coated plate, lyophilized standards, detection reagents, wash buffer, substrate, and stop solution.

The kit supports research into cancer cell signaling, where RSK1 is often dysregulated, and translational work on metabolic regulation. Investigators probing EGF, PDGF, or insulin signaling pathways can monitor RSK1 levels across dose-response experiments, time-course studies, and treatment comparisons. Sample handling follows standard cell biology and biochemistry workflows, and results pair well with downstream western blot confirmation of pathway activation.

Each kit contains reagents for one microplate, enabling full standard curves alongside multiple biological replicates in a single experiment.