Human Ribophorin I (RPN1) ELISA Kit

This Sandwich ELISA from Assay Genie quantifies human Ribophorin I (RPN1), an essential 68 kDa subunit of the oligosaccharyltransferase (OST) complex anchored in the endoplasmic reticulum membrane. RPN1 functions as a ribosome-binding acceptor that positions nascent polypeptides for N-linked glycosylation, making it a relevant target for studies of glycoprotein biosynthesis, secretory pathway quality control, and congenital disorders of glycosylation. Researchers investigating ER stress, the unfolded protein response, tumor-associated glycosylation changes, and OST complex assembly frequently use this assay in cell and tissue lysate workflows. The kit provides a pre-coated microplate, lyophilized standards, biotinylated detection antibody, and streptavidin-HRP conjugate, and is run with 100 µL of sample per well. Reconstituted reagents remain active through limited freeze-thaw cycles; unopened components are stored refrigerated and protected from light. For research use only; not intended for diagnostic procedures.