Human Ribonuclease III, Nuclear (RNASEN) ELISA Kit

Used to quantify the nuclear microRNA-processing enzyme DROSHA (also known as RNASEN or Ribonuclease III), this ELISA kit from Assay Genie provides a research tool for measuring enzyme abundance in human tissue homogenates and cell lysate preparations.

DROSHA performs the initial endonucleolytic cleavage of primary microRNA transcripts into precursor miRNAs inside the nucleus, so its protein levels directly influence cellular miRNA output. Researchers studying miRNA biogenesis, post-transcriptional gene regulation, and the upstream control of microRNA networks use this assay to monitor DROSHA abundance across experimental conditions and disease states.

The kit is configured for the sample matrices typically produced during cell fractionation and nuclear protein extraction workflows. By generating a quantitative readout of nuclear RNase III levels, the assay supports comparisons between normal and diseased tissues, surveys of miRNA-processing capacity in cancer cell lines, and tracking of DROSHA dynamics during differentiation, cellular stress responses, or manipulation of pluripotency factors.

Applications include cancer biology studies of dysregulated microRNA-processing machinery in solid tumors, stem cell research into pathway components, and characterization of gene-regulatory networks that depend on nuclear RNase III activity. The product is supplied as a complete ELISA package, intended for research use only and not for diagnostic procedures.