Human Ribonuclease 7/RNASE7 ELISA Kit

Assay Genie's double-antibody sandwich ELISA kit is designed for colorimetric quantification of human Ribonuclease 7 (RNase7), a protein with antimicrobial activity linked to defense against pathogens, immune responses, and inflammatory processes.

Validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, the assay has a sensitivity of 0.938 ng/mL and a working range of 1.563–100 ng/mL. Documented linearity covers serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions, with average recovery values of 94% (serum), 98% (EDTA plasma), and 98% (heparin plasma). Unopened kit storage is at 2–8°C for 12 months. The target protein corresponds to UniProt entry Q9H1E1, with synonyms including RNase 7, SAP-2, and Skin-derived antimicrobial protein 2.

The procedure involves equilibrating reagents, a 90-minute primary incubation at 37°C, a 60-minute biotin-labeled detection antibody incubation, a 30-minute HRP-Streptavidin binding step, TMB color development in the dark for 10–20 minutes, and absorbance readout at 450 nm after stop solution addition. Provided components include a dismountable pre-coated microplate, lyophilized standard, biotin-labeled antibody (100X), HRP-Streptavidin conjugate (SABC, 100X), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, wash buffer (25X), plate sealer, and a technical manual.