Human RHOF ELISA Kit (HUFI08541)

RHOF (Gene ID 54509; UniProt Q9HBH0; HGNC:15703) maps to chromosomal locus 12q24.31 and encodes a 211-residue small GTPase with a calculated molecular weight of approximately 24 kDa. Aliases RIF and ARHF are tracked by the HUGO Gene Nomenclature Committee alongside the official symbol RHOF (ras homolog family member F, filopodia associated). Beyond the canonical role in dorsal filopodia, peer-reviewed work in lung adenocarcinoma models has placed Rif at the center of a Wnt5a-Ror1-Dvl2 signaling axis that supports polarized protrusion formation on Matrigel, front-rear polarity, and invasive migration. The protein is documented at the plasma membrane and in extracellular exosomes, and acts cooperatively with CDC42 and RAC1 to broaden the diversity of actin-based morphologies. Assay Genie's ELISA configuration is built around a pre-coated microplate that holds 96 Assays and is paired with calibrated ng/mL standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash and substrate buffers, and a plate sealer. Reconstituted standard material should be aliquoted and held frozen to limit freeze-thaw cycles, and the unopened kit retains activity for six months at 4 °C from the date of shipment. Intended for research use only in cytoskeletal, cell motility, and oncology-related workflows.