Human Rho Associated Coiled Coil Containing Protein Kinase 1 (Rock1) ELISA Kit

Quantitative measurement of ROCK1 in human research samples is the core purpose of this Assay Genie ELISA kit. ROCK1 (Rho-associated coiled-coil containing protein kinase 1) is a serine/threonine kinase downstream of RhoA GTPase that governs actin cytoskeleton remodeling, focal adhesion assembly, smooth muscle contraction, and stress fiber formation. Aberrant ROCK1 activity is associated with cardiovascular disease, fibrosis, neurodegeneration, and metastatic cancer progression, making precise protein quantification valuable in cell signaling and translational studies.

This 96-assay kit accommodates a broad range of human-derived specimens, including tissue homogenates, cell lysates, cell culture supernatants, serum samples, plasma samples, and other biological specimens. Each plate is supplied with pre-coated wells, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, and calibrated standards that establish a defined working concentration range. The standard protocol supports reproducible quantification against the included standard curve using a standard microplate reader.

For research use only, this Assay Genie reagent set ships with a complete protocol, wash buffer concentrate, assay diluents, and stop solution. Components should be stored refrigerated upon arrival to preserve activity, with reconstituted standards handled according to the package insert. For laboratories investigating cytoskeletal dynamics, vascular remodeling, or oncology signaling pathways, the kit provides a reliable tool for quantifying ROCK1 protein levels across diverse experimental conditions.