Human Reverse TriiodothyroninerT3 ELISA Kit

Designed for the quantitative measurement of Reverse Triiodothyronine (rT3), this competitive ELISA kit from Assay Genie is coated with antigen and configured for use with human samples. Accepted sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay has a reported sensitivity of 56.25 pg/mL and a working range of 93.75-6000 pg/mL. Kits should be stored sealed at 2-8°C and not cryopreserved.

Reverse Triiodothyronine is a biologically inactive form of thyroid hormone implicated in the regulation of metabolism and energy balance, with altered rT3 levels linked to thyroid disorders and metabolic syndrome, making it a useful marker in cell biology research. The workflow uses a biotin-labeled antibody with HRP-streptavidin detection and TMB substrate, with absorbance measured at 450 nm on a microplate reader.