Human REPS1 (RalBP1-associated Eps domain-containing protein 1) ELISA KIT (HUFI04911)

The REPS1 protein (RalBP1-associated Eps domain-containing protein 1) is a cytoplasmic adaptor that participates in receptor-mediated endocytosis and downstream signaling cascades. This quantitative immunoassay from Assay Genie is configured to quantify human REPS1 in serum or plasma specimens, providing a tool for laboratories studying endocytic trafficking, growth factor receptor downregulation, and signaling pathways linked to RalBP1.

The kit is supplied as a complete 96 Assays package containing a pre-coated microplate, calibrators, detection reagents, wash buffer, and substrate sufficient to run full standard curves alongside unknown samples. The protocol uses a two-antibody capture approach in which REPS1 present in the specimen is immobilized on the plate and subsequently bound by a labeled reporter antibody, producing a chromogenic signal whose intensity correlates with analyte concentration. Plates are read on a standard microplate reader and concentrations are calculated against the supplied recombinant REPS1 standard curve.

Unopened components should be kept refrigerated; reconstituted standards and detection antibody should be aliquoted and frozen, with limited freeze-thaw exposure to preserve activity. The product is labeled for research use only and is not intended for clinical diagnostic procedures.