Human Regenerating Islet Derived Protein 4 (REG4) ELISA Kit

Studies of gastrointestinal regeneration, inflammatory bowel disease, and GI tract oncology often turn to quantitative measurement of Regenerating Islet Derived Protein 4 (REG4) in patient and experimental samples. This ELISA kit from Assay Genie supports such research by providing reagents in a 96-test microplate format for quantification of REG4 in cell culture supernatant, tissue homogenate, and human biofluid specimens.

Each kit includes pre-coated microwell strips, a biotinylated detection antibody, recombinant REG4 standard for curve generation, streptavidin-enzyme conjugate, wash and dilution buffers, chromogenic substrate, and stop solution. After sample addition and washing steps, absorbance values are read on a standard plate reader. Reagents should be stored refrigerated upon receipt to preserve activity across multiple freeze-thaw cycles of working aliquots.

For assay validation, expected evaluations include intra- and inter-plate coefficient of variation, dilution linearity, and recovery in spiked experimental matrices. Researchers may also confirm specificity by testing REG4-depleted samples or unrelated lectin family members. The kit is intended for research use only and is not validated for clinical diagnostic decision-making.

Investigators studying EGFR-AKT or STAT3 signaling pathways, where REG4 has documented downstream effects, or those screening patient cohorts for prognostic biomarker work, will find this assay a practical readout tool.