Human RBX1 ELISA Kit (HUFI06864)

The Assay Genie RBX1 quantification kit is a research-use immunoassay that measures endogenous human RING-box protein 1, the catalytic RING finger subunit of Cullin-RING E3 ubiquitin ligase complexes that drives substrate ubiquitination and proteasomal turnover. Researchers focused on neddylation biology, CRL cycle dynamics and CAND1-mediated remodeling, or on RBX1 overexpression in hepatocellular and lung tumor models and chemoresistance pathways can apply the kit to clarified human cell lysates, tissue homogenates, and circulating blood fractions. Each kit is supplied with a pre-coated microwell plate, lyophilized calibrator standards, biotinylated detection antibody, streptavidin-HRP conjugate, concentrated wash buffer, TMB substrate, and stop solution, supporting the full plate format for one complete run on a standard microplate reader at the recommended absorbance wavelength. Reconstituted stock reagents should be kept cold and shielded from light, with unused microwell strips resealed in the foil pouch with desiccant; standards should be aliquoted into single-use portions to avoid repeated freeze-thaw cycles. The full workflow fits inside a half-day, with optional overnight sample incubation when maximal lower-end resolution is required. Intended for laboratory research use only, not for diagnostic procedures.