Human RBP4/Retinol-Binding Protein 4 ELISA Kit

Assay Genie provides a high-quality Sandwich ELISA kit for the quantitative measurement of Retinol-Binding Protein 4 (RBP4), a key transporter of retinol (vitamin A) in the bloodstream. Designed for human samples, the assay delivers high sensitivity and specificity, making it a valuable research tool for investigating metabolic processes and conditions such as diabetes, obesity, and cardiovascular diseases.

Using a colourimetric double-antibody sandwich format, the kit offers a sensitivity of 0.938 ng/mL and a working range of 1.563–100 ng/mL. It is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with components stored at 2–8°C for up to 12 months. The procedure involves equilibrating reagents and TMB substrate, loading standards and samples for a 90-minute incubation at 37°C, biotin-labelled detection antibody binding, HRP-streptavidin binding, colour development, and absorbance measurement at 450 nm.