Human RBP3 ELISA Kit

Manufactured by Assay Genie, this colorimetric Sandwich ELISA (Double Antibody) is designed for the accurate quantification of Retinol-Binding Protein 3 (RBP3) in human samples. RBP3 is a vital protein involved in the transport of retinol (vitamin A) and participates in processes including vision, immune function, and cell growth. The assay uses a pre-coated microplate with absorbance read at 450 nm following TMB substrate development.

The kit is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types. Linearity data is provided, with serum linearity of 86-99% at a 1:2 dilution cited as an example, and overall recovery ranging from 85-104% across tested matrices. Reactivity is specified for human samples only.

Reagents are stored at 2-8°C for 12 months. The workflow follows a standard sandwich protocol: primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding, HRP-Streptavidin interaction, and color development with TMB in the dark for 10-20 minutes. The kit supports research into RBP3 dysregulation associated with vitamin A deficiency, liver disease, and diabetes, where it serves as a valuable biomarker.