Human RBCK1 (RanBP-type and C3HC4-type zinc finger-containing protein 1) ELISA KIT (HUFI04913)

The Assay Genie RBCK1 immunoassay kit enables quantitative measurement of RanBP-type and C3HC4-type zinc finger-containing protein 1 in human research specimens. RBCK1, also known as HOIL-1L, is a critical E3 ubiquitin ligase that functions as part of the linear ubiquitin chain assembly complex (LUBAC) alongside HOIP and SHARPIN, where it contributes to the generation of linear (M1-linked) ubiquitin chains that regulate NF-κB signaling and downstream inflammatory responses. The target is of considerable interest in studies of innate immunity, cytokine-driven signaling cascades, and selective autophagy, and it has been investigated in the context of viral immune evasion strategies, autoinflammatory disease mechanisms, and cellular responses to oxidative stress. Researchers studying the LUBAC-associated regulatory axis, including its crosstalk with canonical and non-canonical NF-κB pathways, will find this reagent useful for profiling relative protein levels across experimental cohorts. The kit is supplied in a 96 Assays configuration and is intended for research use only. Specimen handling recommendations call for refrigerated storage of reagents and pre-coated components prior to use, with all kit components equilibrated to room temperature before initiating the assay protocol.