Human RBBP9 ELISA Kit (HUFI06896)

Quantitative immunoassay from Assay Genie for measuring retinoblastoma-binding protein 9 (RBBP9) concentrations in human serum or plasma specimens. RBBP9 is a serine hydrolase that interacts with the retinoblastoma tumor suppressor (pRb) and modulates E2F-dependent transcription, with documented involvement in pancreatic ductal adenocarcinoma and other epithelial malignancies where altered Rb pathway signaling is observed. The protein is encoded on chromosome 20q11.23 and has been studied in the context of chromatin remodeling, apoptosis regulation, and cancer cell proliferation.

Each kit supplies pre-coated microplate strips, biotinylated detection antibody, streptavidin-HRP conjugate, calibration standards, and substrate sufficient for 96 individual determinations. Reconstituted reagents should be handled according to the supplied insert; aliquoting after reconstitution is recommended to limit repeated freeze-thaw exposure of working standards, and shelf-life claims on the lot certificate apply when the sealed kit is held refrigerated between uses.

Intended for research use only and validated for in vitro measurement of the human ortholog, the assay enables investigators studying Rb/E2F pathway dysregulation, chromatin-associated hydrolase activity, and tumor biomarker discovery to quantify circulating RBBP9 levels with specificity to human samples. Typical workflows include oncology research, biomarker screening in matched serum or plasma cohorts, and mechanistic studies of pRb-binding partners in cell lysate or clinical tissue preparation. Researchers comparing tumour versus normal circulating levels, or probing response to candidate therapeutics that perturb Rb pathway signaling, will find this format consistent with photometric ELISA workflows used across translational laboratories.