Human RB1CC1 ELISA Kit (HUFI08564)

The Assay Genie RB1CC1 ELISA Kit enables quantitative measurement of RB1CC1, also known as FIP200 (FAK-family interacting protein of 200 kDa), a key regulator of the ULK1 autophagy initiation complex. Use this assay to investigate autophagy flux, nutrient sensing pathways, and tumor suppressor signaling in human research samples.

RB1CC1 functions as a scaffold within the ULK1-ATG13-FIP200 complex, coordinating mTOR-dependent phosphorylation events that govern autophagosome formation. Loss-of-function studies have linked RB1CC1 mutations to familial breast cancer susceptibility, making quantitative measurement valuable for oncology and cell biology research. The kit offers human-specific detection of endogenous RB1CC1 levels for translational research applications.

Each kit provides a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, and dilution buffers. Researchers should equilibrate reagents to room temperature before use and avoid repeated freeze-thaw cycles of prepared working solutions. For research use only; not intended for diagnostic procedures.