Human RASGRF1 ELISA Kit (HUFI06901)

RASGRF1 (Ras protein-specific guanine nucleotide releasing factor 1) is a neuronal Ras-GEF that links calmodulin and G-protein coupled receptor signals to MAPK cascade activation, placing it at the center of studies on synaptic plasticity, long-term memory formation, and Ras-driven oncogenesis. This quantitative ELISA from Assay Genie allows laboratories to measure endogenous human RASGRF1 protein in research specimens, supporting neuroscience, signal transduction, and tumor biology investigations.

The kit is supplied as a microplate-based two-antibody immunoassay with a pre-coated plate, lyophilized recombinant human RASGRF1 standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash concentrate, chromogenic substrate, and stop reagent. Researchers typically load 100 uL of prepared sample or standard per well, then proceed through sequential binding, washing, and color development steps. Optical density values are read on a standard plate reader for quantitative interpolation against the standard curve.

Validated specifically for human reactivity, with cross-reactivity against other species not characterized by the manufacturer. Intended for research use only, not for clinical diagnostic decision-making. Components should be stored refrigerated upon arrival, kept sealed between runs, and protected from direct light to preserve antibody affinity and HRP conjugate activity through the printed expiration date.