Human RASGEF1B ELISA Kit (HUFI06899)

This Assay Genie kit enables quantitative measurement of RASGEF1B, a Ras-specific guanine nucleotide exchange factor implicated in MAPK signaling cascades across immune cell populations. The assay supports investigation of RASGEF1B expression during macrophage activation, dendritic cell maturation, and B-cell receptor signaling events.

RASGEF1B has emerged as a candidate biomarker in inflammatory disease research, with published studies examining its role in rheumatoid arthritis, autoimmune disorders, and tumor immunology. The species-specific capture antibody is validated against human RASGEF1B epitopes, supporting in vitro investigations of GTPase signaling pathways in cell culture models, primary lymphocytes, and tissue homogenates.

Each kit includes a pre-coated microplate with capture antibodies, biotinylated detection reagent, streptavidin-HRP conjugate, and lyophilized standards for generating calibration curves. Storage at 4 degrees C preserves reagent stability, and the assay produces absorbance readings compatible with routine microplate readers used in immunology and cell biology laboratories.