Human Ras Association Domain Containing Protein 1 (RASSF1) ELISA Kit

The RASSF1 protein functions as a tumor suppressor and is frequently inactivated by promoter hypermethylation across multiple cancer types, including non-small cell lung carcinoma, breast carcinoma, and clear cell renal carcinoma. Quantitative measurement of RASSF1 expression therefore supports research in cancer epigenetics and biomarker discovery. This immunoassay from Assay Genie is calibrated for use with human-derived specimens, including venous blood fractions and prepared tissue lysates, fitting common laboratory sample handling workflows. The kit is supplied in a 96-assay plate layout and produces an absorbance-based readout on standard microplate equipment. All reagents are packaged in individual vials for reconstitution and use per the enclosed protocol, and the assay is designated for in vitro research use only. Investigators studying Hippo pathway signaling, where RASSF1 modulates MST1/2 kinase activity and downstream YAP1 phosphorylation events, can integrate the kit into studies of cell-cycle regulation, apoptosis induction, and microtubule stability. Each kit supports standard ELISA workflows including standard curve generation, sample incubation steps, and concentration determination from test specimens.