Human RAR Related Orphan Receptor Alpha (RORa) ELISA Kit

RORα (retinoic acid receptor-related orphan receptor alpha) is a nuclear receptor transcription factor with documented roles in circadian rhythm regulation, cerebellar Purkinje cell development, and lipid/glucose homeostasis. Quantifying this protein in experimental samples is a recurring requirement in chronobiology, neurobiology, and metabolic disease research, where altered RORα signaling has been linked to tumor suppression pathways and circadian gene programs.

The Assay Genie quantitative immunoassay is configured as a 96-test microplate kit, providing sufficient materials for moderate-throughput cohort screening. Each pack includes pre-coated assay wells, biotinylated detection reagent, enzyme conjugate, wash buffer concentrate, chromogenic substrate solution, and stop reagent, along with reference standards for standard curve generation and a detailed protocol insert.

Intended strictly for research use only, the kit is not validated for clinical diagnostic procedures. Reagents should be stored refrigerated upon arrival; thaw frozen components only as needed and avoid repeated freeze-thaw cycles to preserve antibody binding activity. Allow plates and buffers to equilibrate to room temperature before initiating the assay workflow.

Investigators commonly apply this kit to characterize RORα expression dynamics in cell culture models, freshly isolated tissue samples, and biofluids relevant to nuclear receptor signaling studies. The protocol supports an absorbance-based readout on conventional microplate readers commonly found in research laboratories.