Human RAP1B (Ras-related protein Rap-1b) ELISA Kit (HUFI08569)

Integrin signaling, focal adhesion turnover, and platelet biology research rely on accurate quantification of small GTPases that act as molecular switches between active and inactive states. Ras-related protein Rap-1b (RAP1B) is one such 21 kDa GTP-binding regulator of cell migration, cytoskeletal remodeling, and immune cell trafficking. Assay Genie supplies a 96 Assays-format paired-antibody ELISA that delivers an absorbance-based read-out for measuring endogenous RAP1B in prepared laboratory specimens.

Capture antibodies are pre-bound to the wells of the supplied plate, and a biotinylated detection reagent combined with streptavidin-HRP generates a chromogenic signal proportional to the antigen captured. A recombinant human RAP1B standard is included in serial dilution to support standard curve generation, with user samples interpolated against the fitted curve. Reconstituted working solutions should be stored refrigerated between experimental sessions to preserve antibody binding activity.

The reagents are intended for in vitro research use only and are appropriate for serum or plasma specimens handled under standard biosafety precautions. Each package contains the coated well plate, detection antibody, recombinant standards, streptavidin-HRP conjugate, wash buffer concentrate, substrate solution, and a printed protocol with recommended dilution guidelines and assay performance characteristics.