Human RANK PharmaGenie ELISA Kit (SBRS1030)

Osteoclast biology and bone remodeling research depend on precise quantitation of soluble human RANK (Receptor Activator of Nuclear factor Kappa-B, TNFRSF11A), and this Assay Genie PharmaGenie kit provides plate-based ELISA measurement of that receptor in serum specimens, plasma specimens, and cell culture supernatant samples. The RANK/RANKL/OPG signaling axis governs osteoclast differentiation, dendritic cell maturation, and tumor necrosis factor receptor pathway activity, so investigators studying osteoporosis, rheumatoid arthritis, and metabolic bone disease characterize receptor levels in clinical research samples using this quantitative assay. Each kit ships with a capture microplate, biotinylated detection reagent, streptavidin-HRP conjugate, lyophilized standard vials, chromogenic substrate, stop solution, and wash buffer, supporting 96 sample determinations per kit. Standards are reconstituted and aliquoted before freezing; working aliquots should be stored refrigerated and protected from light to limit freeze-thaw cycles on the chromogenic substrate and conjugate. Signal intensity is proportional to RANK concentration, measured on a standard microplate reader. The kit is calibrated specifically for human RANK, applicable to in vitro cell culture experiments, immunohistochemistry companion workflows, and translational research programs labeled for research use only.