Human RalA Binding Protein 1 (RALBP1) ELISA Kit

RALBP1 (also called RLIP76) sits at the junction of receptor endocytosis and ATP-dependent xenobiotic transport, shuttling glutathione and glucuronide conjugates out of cells while participating in clathrin-coated vesicle trafficking. The Human RalA Binding Protein 1 (RALBP1) ELISA Kit from Assay Genie provides research laboratories a quantitative two-site immunoassay for measuring this multifunctional effector of RalA small GTPase in tissue homogenates and cell lysates prepared from human samples.

Encoded by the RALBP1 gene on chromosome 18p11.22 and expressed as a ~76 kDa polypeptide, RALBP1 carries intrinsic ATPase activity and acts as a GTPase-activating protein for Rac1 and Cdc42, linking endocytic trafficking to cytoskeletal remodeling and MAPK signaling. RALBP1 has drawn sustained research interest because of its overexpression in multiple carcinomas and because its conjugate-transport activity contributes to multi-drug resistance phenotypes, including reduced cellular response to doxorubicin and other chemotherapeutics. Investigators apply this kit to compare RALBP1 levels between matched tumor and adjacent normal tissue, to monitor changes after siRNA knockdown or pathway perturbation, and to correlate protein abundance with apoptosis, efflux, or signaling readouts. Each kit provides reagents sufficient for up to 96 determinations on a standard microplate, with results obtained using a conventional ELISA plate reader. For research use only, the assay supports oncology, pharmacology, and Ral-GTPase signaling studies.