Human Rad51D ELISA Kit (HUFI06935)

This quantitative immunoassay from Assay Genie enables researchers to measure RAD51D protein levels in human biological specimens, supporting studies of DNA double-strand break repair and homologous recombination. RAD51D functions as a paralog of the RAD51 recombinase and contributes to maintaining genomic stability, particularly during error-prone DNA replication and in the cellular response to replication stress. Loss-of-function variants in RAD51D have been linked to hereditary breast and ovarian cancer predisposition, making reliable quantification of this protein valuable for oncology research, biomarker development, and mechanistic investigations of the Fanconi anemia and BRCA pathway networks.

The kit includes a pre-coated microplate, detection antibodies, standard recombinant RAD51D protein, and substrate reagents sufficient for 96 determinations. Upon receipt, reagents should be refrigerated and protected from light to preserve activity until first use. Researchers commonly apply this assay to compare RAD51D expression between tumor and matched normal tissues, to assess induction of the protein following DNA-damaging treatments, and to screen compound libraries for modulators of homologous recombination. The kit is intended for research use only and should be performed by trained laboratory personnel using a standard microplate reader capable of measuring absorbance at an appropriate detection wavelength.