Human RAD51B (DNA repair protein RAD51 homolog 2) ELISA Kit (HUFI06922)

This RAD51B quantification kit from Assay Genie supports research into homologous recombination and DNA double-strand break repair. RAD51B, also called RAD51L1, is one of five RAD51 paralogs (RAD51B, RAD51C, RAD51D, XRCC2, XRCC3) and functions within the BCDX2 sub-complex with RAD51C to promote error-free repair of damaged DNA in the nucleus. Antibodies are raised against the human RAD51B sequence, distinguishing the target from its paralog family members.

Researchers studying tumor biology apply this assay to investigate RAD51 paralog expression in breast, ovarian, and other cancers, as well as mechanisms of chemotherapy resistance and Fanconi anemia pathway function. The microplate format integrates with standard laboratory workflows, complementing Western blot, PCR, and immunohistochemistry for protein and gene expression analysis.

Common study designs include stratifying patient cohorts by DNA repair capacity, profiling RAD51 paralog levels across cell line panels, and supporting biomarker discovery on genomic instability. Reconstituted reagents should be aliquoted and stored to limit freeze-thaw cycles, and protected from light to preserve antibody binding activity. The kit is intended for research use only.