Human RAD51 Homolog (RAD51) ELISA Kit

Quantify endogenous human RAD51 protein with this research-use-only immunoassay from Assay Genie. RAD51 is the central eukaryotic recombinase that drives homologous strand invasion during DNA double-strand break repair, supporting studies of genome stability, BRCA1/2 pathway function, and tumor cell responses to genotoxic stress. Each kit includes a pre-coated microplate plus calibrator, detection antibody, and substrate reagents sufficient for the standard curve and unknown samples. The protocol uses a 100 µL sample volume, with sequential incubation steps totaling roughly 3.5 hours at 37 °C and a final stop reaction read on a standard plate reader. Unopened components should be stored at 4 °C and protected from light; reconstituted standards and biotinylated detection reagent should be aliquoted and held frozen to limit freeze-thaw cycles. Investigators in Fanconi anemia, PARP inhibitor mechanism-of-action, and hereditary breast cancer research commonly pair quantitative RAD51 readouts with immunofluorescence foci analysis.