Human RAD23A ELISA Kit (HUFI06931)

Quantitative measurement of UV excision repair protein RAD23A in human research specimens is enabled by this colorimetric immunoassay from Assay Genie. RAD23A serves as a recognition factor in the nucleotide excision repair pathway and bridges ubiquitinated substrates to the 26S proteasome, making the assay relevant for DNA damage response studies, genome stability research, and investigations of ubiquitin-proteasome crosstalk. The kit uses a biotin-streptavidin-HRP detection workflow on a 96 Assays microplate, compatible with standard absorbance plate readers. It is calibrated for use with clarified human serum, EDTA-treated plasma specimens, and lysate supernatants from cultured human cell lines. Each kit contains pre-coated microwell strips, lyophilized recombinant RAD23A standard, biotinylated detection antibody, streptavidin-HRP conjugate, wash concentrate, TMB substrate, and stop solution. Standard dilutions generate a calibration curve for interpolating sample concentrations, with all reagents intended for research use only. Components should be refrigerated upon arrival and equilibrated to room temperature before use to ensure reproducible binding across runs. The kit supports laboratory workflows in molecular oncology, photobiology, and DNA repair biomarker discovery, providing researchers a reliable tool for measuring RAD23A protein dynamics across experimental conditions.