Human RAD17 ELISA Kit (HUFI06929)

Built for quantitative measurement of the RAD17 cell cycle checkpoint protein in human specimens, this assay from Assay Genie supports research into DNA damage response pathways and genomic stability. RAD17 functions as a key component of the 9-1-1 clamp-loading complex that activates the ATR-Chk1 signaling axis following replication stress or DNA lesions, making its quantification valuable for studies of cell cycle regulation, genotoxicity screening, and tumor biology.

The kit is configured for 96 Assays and provides the core reagents needed for the procedure, including antibody-coated microplate wells, detection antibodies, and standard calibrators. Intended for research use only, the reagents enable investigation of RAD17 expression dynamics in experimental samples relevant to chemotherapy response, radiation biology, and inherited genome instability disorders linked to DNA repair defects.

Laboratory workflows begin with the application of prepared samples to antibody-coated wells, followed by incubation, washing, and addition of enzyme-conjugated detection reagent. A substrate step produces a measurable signal proportional to RAD17 concentration, quantified against the standard curve. Researchers studying checkpoint signaling often pair RAD17 measurements with complementary readouts such as p-Chk1, γ-H2AX, or RPA32 phosphorylation to characterize the broader DNA damage response cascade.