Human RacGAP1 ELISA Kit

From Assay Genie, this sandwich ELISA kit is designed for the precise measurement of RACGAP1 (Rac-GTPase Activating Protein 1) levels in human samples using a colorimetric double-antibody detection method. The kit is validated for serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples, and is suited to research in cell cycle biology. RACGAP1 is a key protein involved in cell division and migration, with roles in cytokinesis and cell adhesion, and its dysregulation has been implicated in cancer and inflammatory disorders. The associated Uniprot ID is Q9H0H5.

Performance specifications include a sensitivity of 46.875 pg/mL and a standard range of 78.125-5000 pg/mL, with the kit stable for 12 months when stored at 2-8°C. Linearity was demonstrated for serum at 91-104%, 87-103%, and 87-98% (1:2, 1:4, and 1:8 dilutions, n=5), EDTA plasma at 85-97%, 82-101%, and 82-98%, and heparin plasma at 81-99%, 85-98%, and 85-95%. Spike-and-recovery testing showed average recoveries of 96% for serum, 91% for EDTA plasma, and 98% for heparin plasma (n=5).

The workflow involves equilibrating reagents to room temperature, loading standards and samples onto the pre-coated plate, and incubating at 37°C. Detection uses a biotin-labeled antibody followed by HRP-Streptavidin conjugate, with TMB substrate for color development and absorbance read at 450 nm.