Human RAB11FIP3 ELISA Kit (HUFI08582)

The Assay Genie RAB11FIP3 immunoassay is configured for quantitative measurement of RAB11FIP3 in human research samples, supporting investigation of Rab11-mediated endosomal recycling and cytokinesis. The kit ships as a complete 96 Assays package containing pre-coated microplate wells, biotinylated detection antibody, streptavidin-HRP conjugate, lyophilized protein standard, wash buffer, substrate solution, and stop reagent.

RAB11FIP3 (Rab11 Family Interacting Protein 3) functions as a downstream effector of Rab11a and Rab11b GTPases, coordinating return of internalized cargoes from pericentrosomal recycling endosomes to the plasma membrane. The protein is also recruited to the midbody during the abscission stage of cytokinesis, linking membrane trafficking to the final separation of dividing cells. Investigators studying vesicle trafficking, endosome maturation, or cell division use this kit to monitor RAB11FIP3 abundance in cell lysates and clarified culture supernatants from in vitro cell culture models.

Each plate includes a standard curve prepared by serial dilution, and absorbance is read on a standard microplate reader. The assay is intended for research use only and is not validated for diagnostic or therapeutic decision-making.

Store unopened kits refrigerated at 4 °C and protect from light. Reconstituted standards and working-strength detection antibody should be aliquoted into single-use vials and frozen at -20 °C to preserve activity across repeated freeze-thaw cycles.