Human R-Spondin 3 (RSPO3) ELISA Kit

RSPO3 (R-Spondin 3) is a secreted cysteine-rich protein that potentiates canonical Wnt/β-catenin signaling by engaging LGR4, LGR5, and LGR6 receptors and blocking ZNRF3/RNF43-mediated turnover of Frizzled receptors at the plasma membrane. The Assay Genie kit supports research in vascular and endothelial biology, osteoblast differentiation, intestinal crypt stem cell maintenance, and tumor microenvironment studies, and integrates readily with LGR5+ organoid culture protocols where RSPO3-conditioned media is a standard supplement. The biotin–streptavidin-HRP detection chemistry enables quantification in conditioned media from cultured cells, including colorectal carcinoma, osteosarcoma, and endothelial lines, where RSPO3 secretion is often used as a read-out of Wnt pathway activity. Researchers typically pair the ELISA with downstream qPCR of Wnt target genes such as AXIN2, LGR5, or ASCL2, and intra-assay variability is reported below 10% CV across replicate wells.