Human PYY PharmaGenie ELISA Kit (SBRS1028)

Peptide YY (PYY) is a 36-amino-acid gastrointestinal hormone secreted by enteroendocrine L-cells of the distal ileum and colon, with circulating levels rising after meals to modulate satiety, gastric emptying, and energy balance. The Assay Genie Human PYY PharmaGenie ELISA Kit is a quantitative enzyme immunoassay that enables researchers to measure PYY concentrations in human research specimens.

Scientists investigating appetite regulation, bariatric intervention outcomes, type 2 diabetes pathophysiology, and gut-brain signaling pathways rely on this kit to quantify circulating PYY in studies of metabolic disease. The color-based readout is read on a standard microplate reader compatible with common laboratory equipment, and each kit supplies reagents and antibody-coated wells for 96 individual determinations.

Validated specimen types include fractions prepared from human blood as well as conditioned media from cultured cells, enabling both clinical sample analysis and in vitro cell-based experiments. Diluted samples and reconstituted standards are added to pre-coated wells, followed by a biotinylated detection antibody and streptavidin-HRP conjugate, with absorbance read at the appropriate wavelength after stop solution addition.

Reagents should be stored refrigerated upon arrival and protected from light to maintain antibody binding activity through the kit's shelf life. The product is supplied for research use only and is not intended for diagnostic procedures.