Human Pyruvate Dehydrogenase Phosphatase (PDP) ELISA Kit

The Human Pyruvate Dehydrogenase Phosphatase (PDP) ELISA Kit from Assay Genie quantifies PDP, a mitochondrial serine/threonine phosphatase that activates the pyruvate dehydrogenase complex (PDC) by dephosphorylating Ser293, Ser300, and Ser232 on the E1α subunit. Because PDC gates the conversion of pyruvate to acetyl-CoA, PDP measurements are used in research on glucose homeostasis, mitochondrial bioenergetics, and the lactic acidosis phenotypes associated with PDP1 deficiency.

The microplate is pre-coated with capture antibody; dilute standards and add 100 µL of prepared sample per well, then follow the sequential incubation and wash steps. Reconstituted reagents should be stored refrigerated and remain stable through the kit's 12-month shelf life. For research use only, in vitro laboratory measurements.