Human Pyruvate Dehydrogenase Beta (PDHb) ELISA Kit

Quantitative measurement of the pyruvate dehydrogenase E1 component beta subunit (PDHb) in human research samples is supported by this colorimetric immunoassay from Assay Genie. The kit is configured for 96 determinations per plate and is validated for use with tissue homogenates, cell lysates, and cell culture supernatants, making it applicable to metabolic and mitochondrial studies across cell biology and biochemistry workflows.

PDHb forms the catalytic decarboxylase portion of the pyruvate dehydrogenase complex, a mitochondrial multi-enzyme assembly that links glycolysis to the tricarboxylic acid cycle by converting pyruvate to acetyl-CoA. Researchers investigating metabolic dysfunction, lactic acidosis, cancer metabolism, or mitochondrial regulation rely on protein-level quantification of individual subunits to track complex assembly, expression changes, and post-translational modifications in human cell and tissue models.

Each kit is supplied with a pre-coated microplate and the reagents necessary for a standard ELISA workflow. Refrigerated storage is recommended for immunoassay components, and the protocol is intended for research use only. Colorimetric readout enables plate-based analysis on standard absorbance readers in laboratory settings.