Human PYGO2 ELISA Kit (HUFI06946)

The Pygopus Homolog 2 (PYGO2) protein is a nuclear effector of the canonical Wnt/β-catenin signaling cascade, where it partners with BCL9 to recruit chromatin-remodeling complexes and drive transcription of Wnt target genes. By providing a quantitative readout of PYGO2 abundance in clinical and research specimens, this enzyme-linked immunosorbent assay from Assay Genie supports investigation of developmental patterning, stem-cell maintenance, and oncogenic pathways in which Wnt activity is dysregulated.

Configured as a strip plate, the kit accommodates up to 96 Assays per run and is intended for research use only. Investigators working with human-derived specimens can apply the assay to profile baseline PYGO2 levels, compare tumor versus matched normal tissue, or track pathway modulation following pharmacological inhibition of Wnt signaling. Reagents are supplied ready-to-use or as concentrated stocks prepared according to the included protocol, with absorbance measurement performed on a standard microplate reader.

Typical laboratory workflows pair the kit with recombinant PYGO2 standards for generating standard curves, and downstream analyses commonly include normalization to total protein concentration or to housekeeping markers such as β-catenin and BCL9. Storage recommendations provided in the product insert should be observed to preserve antibody activity across multiple freeze-thaw cycles.