Human PYGM/Glycogen Phosphorylase, Muscle ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is a colorimetric assay intended for the quantitative measurement of PYGM (Glycogen Phosphorylase, Muscle) in human samples. It is reactive with human specimens and validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types.

The kit employs a Sandwich ELISA, Double Antibody format with a stated sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL. PYGM (synonyms: Myophosphorylase, Glycogen phosphorylase, muscle form; UniProt P11217) is an enzyme involved in glycogen metabolism, making this kit relevant to research into glycogen storage diseases and muscle disorders.

Reported linearity covers serum (1:2 92–104%, 1:4 83–101%, 1:8 84–94%), EDTA plasma (1:2 85–95%, 1:4 82–91%, 1:8 81–93%), and heparin plasma (1:2 88–101%, 1:4 83–101%, 1:8 80–94%). Recovery ranges were 88–100% (average 92%) for serum, 85–103% (average 93%) for EDTA plasma, and 86–98% (average 91%) for heparin plasma. Storage is 2–8°C for 12 months.