Human PYCARD (Apoptosis-associated speck-like protein containing a CARD) ELISA Kit (HUFI08097)

Assay Genie's PYCARD ELISA Kit enables quantitative measurement of Apoptosis-associated speck-like protein containing a CARD (ASC) in human biological samples. PYCARD is a bipartite adaptor protein harboring both a pyrin domain (PYD) and a caspase activation and recruitment domain (CARD), making it central to inflammasome assembly by linking pattern recognition receptors such as NLRP3 to pro-caspase-1. ASC-driven inflammasome signaling drives maturation of IL-1β and IL-18 and is implicated in autoinflammatory disease, chronic inflammation, pyroptosis, and tumor immunology research. The assay uses a human-specific capture antibody and is provided in a 96-well microplate kit for research use only investigations of caspase-1 activation and downstream cytokine cascades. Unopened reagents should be stored refrigerated as indicated on the package insert, and reconstituted standards should be aliquoted to limit repeated freeze-thaw exposure.