Human Putative pro-MCH-like protein 1 (PMCHL1) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the quantitative measurement of human Putative pro-MCH-like protein 1 (PMCHL1). The assay uses a colorimetric detection method and is reactive with human samples, supporting research into PMCHL1, a protein that belongs to the melanin-concentrating hormone family and is located at chromosomal region 5p14.3.

The kit offers a sensitivity of 42 pg/mL with a detection range of 78-5000 pg/mL, and shows an intra-assay CV of 4.6% and an inter-assay CV of 7.8%. Specificity is for natural and recombinant human PMCHL1. Compatible sample types include serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Recovery is reported at an average of 88% (range 82-94%) for serum and 90% (range 84-96%) for plasma.

Linearity has been demonstrated across dilutions from 1:2 to 1:16. For serum (N=5), recovery ranged from 99-110% at 1:2 to 103-112% at 1:16. EDTA plasma (N=5) showed 87-100% at 1:2 to 103-112% at 1:16, while heparin plasma (N=5) showed 84-95% at 1:2 to 110-120% at 1:16. The associated UniProt accession is Q16048 and the molecular weight is 9,715 Da.