Human Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP) ELISA Kit

This competitive ELISA kit from Assay Genie is designed for the quantitative detection of human Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP) in serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids. The assay is reactive with human samples and is intended for research use only.

The manufacturer's stated performance characteristics include a detection range of 1.56-100ng/mL and a sensitivity of 0.54ng/mL. Reported precision values are an intra-assay CV of 6.2% and an inter-assay CV of 10.6%. Linearity across 1:2 to 1:16 dilutions (N=5) was 96-118% in serum, 87-116% in EDTA plasma and 85-116% in heparin plasma. Average recovery was 95% in serum (range 89-101%) and 97% in plasma (range 91-103%).

Specificity is reported for natural and recombinant human CLPP (UniProt Q16740, NCBI Gene ID 8192), a 24.2kDa (222 amino acid) protease localised to the mitochondrial matrix and functioning as the proteolytic component of the Clp complex in ATP-dependent protein degradation. Storage temperature is -20°C, with shipping at +4°C.