Human Purinergic Receptor P2Y, G Protein Coupled 14 (P2RY14) ELISA Kit

Quantitative measurement of the human purinergic receptor P2Y14 in tissue homogenates, cell lysates, and related biological preparations is delivered through this Assay Genie colorimetric ELISA kit formatted for 96 assays. P2Y14 is a G-protein coupled receptor that responds to UDP-glucose and UDP-galactose, and it has documented involvement in immune cell chemotaxis, hematopoietic stem cell mobilization, airway inflammation, and glial signaling. Investigators in immunology, neurobiology, and respiratory research apply this assay to monitor receptor expression changes following cytokine stimulation, pathogen exposure, or pharmacological modulation. Each kit supplies pre-coated strip wells, lyophilized recombinant P2Y14 standard, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, substrate solution, and stop reagent. Unopened components are shipped and stored at refrigerated temperature to preserve antibody and standard activity; reconstituted reagents should be aliquoted to limit repeated freeze-thaw exposure. The protocol is run on standard microplate equipment with a small microliter sample volume per well, and the absorbance endpoint is read for interpolation against the supplied reference standard. The assay is intended for research use only and has not been validated for clinical diagnostic procedures.