Human PUM2 ELISA Kit (HUFI06959)

Investigate post-transcriptional gene regulation with this quantitative immunoassay from Assay Genie for Pumilio RNA-Binding Family Member 2. PUM2 is a sequence-specific RNA-binding protein of the PUF family that recognizes UGUA(A/U)UAUA motifs in the 3' untranslated regions of target transcripts, typically repressing translation and promoting mRNA decay. These targets govern germline maintenance, neural stem cell self-renewal, and dendritic spine morphogenesis.

Researchers studying spermatogenesis, neurobiology, and tumor biology use this kit to profile PUM2 protein levels in experimental and clinical cohorts. The protocol calls for 100 ul of sample per well, with standards and a chromogenic substrate supplied to support reproducible standard curves. Capture antibody, plates, and detection reagents are included for a complete batch run.

For consistent results, store unopened kit components refrigerated and protect the chromogenic substrate from light. This research use only kit supports studies exploring PUM2 dysregulation in cancer, neurodegeneration, and metabolic disease models.