Human Pulmonary Surfactant Associated Protein A/SPA ELISA Kit

From Assay Genie, this sandwich ELISA is designed for the quantitative detection of Human Pulmonary Surfactant-Associated Protein A (SPA) in human biological samples. The kit uses a colorimetric double-antibody sandwich detection method and is intended for research use in studies of pulmonary surfactant function and respiratory health.

SPA is a vital component of pulmonary surfactant, a substance that reduces surface tension in the lungs and supports proper lung function. The protein also plays a role in immune defense in the lungs, helping protect against infections. Abnormal SPA levels have been linked to various respiratory diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.

The assay is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reported sensitivity is 9.375 pg/mL with a working range of 15.625–1000 pg/mL. The kit should be stored at 2–8°C for 12 months. Linearity and recovery performance is reported for serum, EDTA plasma, and heparin plasma across multiple dilution factors.