Human PUF60 ELISA Kit (HUFI06958)

This assay kit from Assay Genie measures poly(U)-binding splicing factor 60 (PUF60), an RNA-binding protein central to pre-mRNA splicing, polyadenylation, and 3' end processing. Also known as FIR (FUSE-binding protein-interacting repressor) and Ro-BPI, PUF60 resides at chromosome 8q24.3 and contributes to spliceosome assembly alongside other RNA-processing factors.

Research with this reagent supports investigation of PUF60 biology across multiple fields. PUF60 haploinsufficiency underlies Verheij syndrome, a developmental disorder presenting with craniofacial anomalies, cardiac defects, and intellectual disability. The kit also suits oncology work tracking splicing factor dysregulation in tumor specimens and broader studies of RNA metabolism.

Each kit provides reagents configured for 96 individual determinations in a standard microplate layout, giving research laboratories enough material for medium-throughput screening or serial dilution experiments. End-point absorbance values are captured with a conventional microplate reader, allowing compatibility with instrumentation already present in most immunology and molecular biology facilities.

Intended for research use only, the kit supports studies examining spliceosome function, RNA processing mechanisms, and disease-associated changes in PUF60 expression in human-derived samples.