Human PTS ELISA Kit (HUFI06957)

Targeted to6-pyruvoyltetrahydropterin synthase, the second enzyme in the tetrahydrobiopterin (BH4) biosynthesis pathway, this Assay Genie sandwich ELISA supports quantitative work in metabolic and neuroscience research where BH4 availability influences phenylalanine hydroxylase, tyrosine hydroxylase, and nitric oxide synthase activity. The kit is configured for 96 Assays per plate and is intended for use with human serum, plasma, and related biological fluid samples.

According to published specifications for this assay format, the reported detection range is 0.312 to 20 ng/mL, with sensitivity below 0.119 ng/mL, and intra-assay CV at or below 5.8% and inter-assay CV at or below 10.4%. The assay uses a colorimetric readout, and the included reagents cover the immobilized capture antibody, detection antibody, reference standards, and substrate required to generate a standard curve for each run.

Researchers studying hyperphenylalaninemia, DOPA-responsive dystonia, and related BH4-dependent neurotransmitter pathways can use the kit to profile PTS levels in patient-derived serum and plasma. Reagents should be stored refrigerated, equilibrated to room temperature before the assay, and the product is labelled for research use only.