Human PTGES2 ELISA Kit (HUFI06983)

The PTGES2 enzyme, also known as membrane-associated Prostaglandin E Synthase 2 (mPGES-2), converts prostaglandin H2 to prostaglandin E2 and is implicated in inflammatory signaling, reproductive biology, and oncology research. This quantitative immunoassay kit from Assay Genie is configured for 96 Assays and is intended for research use only. Researchers studying eicosanoid pathways can apply the assay to in vitro investigations of PGE2 biosynthesis regulation. Wells are pre-coated with capture antibody specific for human PTGES2, and bound analyte is revealed through an enzymatic reaction read on a standard microplate reader. Each kit package includes a pre-coated plate, lyophilized standards, detection antibody, assay buffer, wash concentrate, substrate solution, and stop reagent sufficient for one full plate run. Provided the unopened kit is kept refrigerated and protected from light, components remain stable through the expiration printed on the label. The product's specimen guidance covers human serum samples along with heparin- or EDTA-derived plasma specimens, and the protocol accommodates both preparations following routine centrifugation. Researchers measuring PTGES2 in cell lysates, tissue homogenates, or conditioned media should validate their matrix independently before drawing quantitative conclusions. The kit supports use in studies of cyclooxygenase and lipocalin-type prostaglandin synthase crosstalk, and is a useful addition to labs profiling eicosanoid-pathway markers across disease models. Components should be equilibrated to room temperature before opening, and reconstituted standards should be aliquoted to limit repeated freeze-thaw cycles.