Human PTGER2/Prostaglandin E Receptor 2 ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the quantitative detection of human PTGER2 (Prostaglandin E Receptor 2), a receptor involved in the cellular response to prostaglandin E that plays a role in inflammation, pain, and immune regulation. The kit uses a double-antibody sandwich format with colorimetric detection and is intended for use with human samples including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

The assay has a sensitivity of 37.5 pg/mL and a working range of 62.5–4000 pg/mL, with reagents stored at 2–8°C for 12 months. Reported recovery averages are 93% for serum, 93% for EDTA plasma, and 94% for heparin plasma. Linearity has been evaluated in serum, EDTA plasma, and heparin plasma across dilution factors of 1:2, 1:4, and 1:8. The procedure involves a 90-minute primary incubation at 37°C, a 60-minute detection antibody binding step, a 30-minute HRP-streptavidin binding step, 10–20 minutes of TMB color development in the dark, and absorbance measurement at 450 nm after adding stop solution. Provided components include a dismountable pre-coated microplate, lyophilized standard, biotin-labeled antibody, HRP-streptavidin conjugate, TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, wash buffer, stop solution, plate sealer, and a technical manual.